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Ion transport mechanisms in cultured bovine corneal endothelial cells

Item Type:Article
Title:Ion transport mechanisms in cultured bovine corneal endothelial cells
Creators Name:Jentsch, T.J., Keller, S.K. and Wiederholt, M.
Abstract:Intracellular potential measurements of confluent monolayers of cultured bovine corneal endothelial cells were used to define passive ion transport processes in these cells. Previous studies (Jentsch et al., J. Membr. Biol. 78:103 (1984); Jentsch et al., J. Membr. Biol. 81:189 (1984] have provided the experimental basis for a cellular model, in which bicarbonate entry across the basolateral membrane is indirectly driven by a Na+/H+-exchanger, which is inhibitable by amiloride (1mM). Bicarbonate and sodium should leave the cell via an electrogenic bicarbonate sodium cotransport, which is inhibitable by the disulfonic stilbene derivates SITS or DIDS. This model is also consistent with results from transendothelial studies. In this paper, we briefly review the evidence we have obtained for this model and demonstrate, that the electrical response to sodium (depolarization upon Na+-removal) is neither due to an inhibition of Na+/K+-ATPase nor explainable in terms of changes in K+-conductance. This is concluded from the observation of these responses in the presence of ouabain (10(-4)M) or barium (1mM).
Keywords:Biological Transport, Biomechanics, Cultured Cells, Cornea, Endothelium, Biological Models, Osmolar Concentration, Potassium, Sodium, Animals, Cattle
Source:Current Eye Research
Publisher:Taylor & Francis
Page Range:361-369
Date:April 1985
Official Publication:https://doi.org/10.3109/02713688509025149
PubMed:View item in PubMed

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