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| Item Type: | Article |
|---|---|
| Title: | Interactions of pH and K+ conductance in cultured bovine retinal pigment epithelial cells |
| Creators Name: | Keller, S.K., Jentsch, T.J., Koch, M. and Wiederholt, M. |
| Abstract: | Passive ion transport properties were studied in confluent monolayers of cultured bovine retinal pigment epithelial cells using intracellular microelectrode technique. The mean stable intracellular (designated by subscript i) potential was -59.1 +/- 0.8 (SE) mV. Extracellular (designated by subscript o) acidification induced a depolarization, whereas alkalinization induced a hyperpolarization. These effects were observed both in bicarbonate-free as well as in HCO3- Ringer (pHo changed by varying [HCO3-]o at constant pCO2). Acidification of pHi (changed by addition and removal of butyrate, CO2 or NH3) also caused a depolarization. Complete removal of HCO3-/CO2 at constant pHo caused a hyperpolarization. K+ transference, checked by applying high K+o, increased with K+o. It decreased with both extra and intracellular acidification and increased with alkalinization. In the presence of Ba2+, voltage reactions to changes in either pHo or pHi were greatly reduced. Depolarization by 40 mM K+ caused a similar reduction. It is suggested that K+ conductance of bovine retinal pigment epithelial cells is reduced by either intra- or extracellular acidification at normal [K+]o. Depolarization by high K+ induces an increase in K+ transference and reduces pH sensitivity. |
| Keywords: | Action Potentials, Ammonium Chloride, Barium, Bicarbonates, Cultured Cells, Electric Conductivity, Hydrogen-Ion Concentration, Mathematics, Pigment Epithelium of Eye, Potassium, Animals, Cattle |
| Source: | American Journal of Physiology |
| ISSN: | 0002-9513 |
| Publisher: | American Physiological Society |
| Volume: | 250 |
| Number: | 1 Pt 1 |
| Page Range: | C124-C137 |
| Date: | January 1986 |
| Official Publication: | http://ajpcell.physiology.org/cgi/content/abstract/250/1/C124 |
| PubMed: | View item in PubMed |
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