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| Item Type: | Article |
|---|---|
| Title: | Completely functional double-barreled chloride channel expressed from a single Torpedo cDNA |
| Creators Name: | Bauer, C.K., Steinmeyer, K., Schwarz, J.R. and Jentsch, T.J. |
| Abstract: | We have performed an electrophysiological analysis of the recently cloned Torpedo marmorata Cl- channel. Functional expression of Cl- channels in oocytes of Xenopus laevis previously injected with cRNA yielded an outward-rectifying current activated by hyperpolarization. Replacement of Cl- with other anions significantly reduced or inhibited the current. Single-channel recordings from cell-attached patches exhibited burst-like Cl- channel activity with rapid fluctuations between three equally spaced substates (0 pS, 9 pS, and 18 pS). The properties of the cloned Cl- channel were almost identical to those of the reconstituted native T. californica Cl- channel and were in full agreement with the predictions of the double-barreled channel model [Hanke, W. & Miller, C. (1983) J. Gen. Physiol. 82, 25-45]. Our results imply that the cloned cDNA codes for the completely functional Torpedo electroplax Cl- channel. |
| Keywords: | Chloride Channels, Chlorides, DNA, Electric Conductivity, Electrophysiology, Ion Channel Gating, Ion Channels, Membrane Potentials, Membrane Proteins, Biological Models, Oocytes, Probability, Torpedo, Animals, Xenopus Laevis |
| Source: | Proceedings of the National Academy of Sciences of the United States of America |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Volume: | 88 |
| Number: | 24 |
| Page Range: | 11052-11056 |
| Date: | 15 December 1991 |
| Official Publication: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC53071/ |
| PubMed: | View item in PubMed |
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