Multimeric structure of ClC-1 chloride channel revealed by mutations in dominant myotonia congenita (Thomsen)

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Item Type:	Article
Title:	Multimeric structure of ClC-1 chloride channel revealed by mutations in dominant myotonia congenita (Thomsen)
Creators Name:	Steinmeyer, K., Lorenz, C., Pusch, M., Koch, M.C. and Jentsch, T.J.
Abstract:	Voltage-gated ClC chloride channels play important roles in cell volume regulation, control of muscle excitability, and probably transepithelial transport. ClC channels can be functionally expressed without other subunits, but it is unknown whether they function as monomers. We now exploit the properties of human mutations in the muscle chloride channel, ClC-1, to explore its multimeric structure. This is based on analysis of the dominant negative effects of ClC-1 mutations causing myotonia congenita (MC, Thomsen's disease), including a newly identified mutation (P480L) in Thomsen's own family. In a co-expression assay, Thomsen's mutation dramatically inhibits normal ClC-1 function. A mutation found in Canadian MC families (G230E) has a less pronounced dominant negative effect, which can be explained by functional WT/G230E heterooligomeric channels with altered kinetics and selectivity. Analysis of both mutants shows independently that ClC-1 functions as a homooligomer with most likely four subunits.
Keywords:	Anion Channel, Muscle, Myotonia, Human Genetics, Voltage-Gated, Animals
Source:	EMBO Journal
ISSN:	0261-4189
Publisher:	Oxford University Press
Volume:	13
Number:	4
Page Range:	737-743
Date:	15 February 1994
Official Publication:	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC394869/
PubMed:	View item in PubMed

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