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| Item Type: | Article |
|---|---|
| Title: | Inhibition of KCNQ1-4 potassium channels expressed in mammalian cells via M1 muscarinic acetylcholine receptors |
| Creators Name: | Selyanko, A.A., Hadley, J.K., Wood, I.C., Abogadie, F.C., Jentsch, T.J. and Brown, D.A. |
| Abstract: | 1. KCNQ1-4 potassium channels were expressed in mammalian Chinese hamster ovary (CHO) cells stably transfected with M1 muscarinic acetylcholine receptors and currents were recorded using the whole-cell perforated patch technique and cell-attached patch recording. 2. Stimulation of M1 receptors by 10 microM oxotremorine-M (Oxo-M) strongly reduced (to 0-10%) currents produced by KCNQ1-4 subunits expressed individually and also those produced by KCNQ2 + KCNQ3 and KCNQ1 + KCNE1 heteromers, which are thought to generate neuronal M-currents (IK,M) and cardiac slow delayed rectifier currents (IK,s), respectively. 3. The activity of KCNQ2 + KCNQ3, KCNQ2 and KCNQ3 channels recorded with cell-attached pipettes was strongly and reversibly reduced by Oxo-M applied to the extra-patch membrane. 4. It is concluded that M1 receptors couple to all known KCNQ subunits and that inhibition of KCNQ2 + KCNQ3 channels, like that of native M-channels, requires a diffusible second messenger. |
| Keywords: | CHO Cells, KCNQ Potassium Channels, Muscarinic Agonists, Oxotremorine, Pat-Clamp Techniques, Potassium Channel Blockersch, Voltage-Gated Potassium Channels, Muscarinic M1 Receptor, Muscarinic Receptors, Chemical Stimulation, Transfection, Animals, Cricetinae |
| Source: | Journal of Physiology |
| ISSN: | 0022-3751 |
| Publisher: | Cambridge University Press |
| Volume: | 522 |
| Number: | Pt 3 |
| Page Range: | 349-355 |
| Date: | 1 February 2000 |
| Official Publication: | https://doi.org/10.1111/j.1469-7793.2000.t01-2-00349.x |
| PubMed: | View item in PubMed |
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