Tissue distribution and subcellular localization of the ClC-5 chloride channel in rat intestinal cells

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Item Type:	Article
Title:	Tissue distribution and subcellular localization of the ClC-5 chloride channel in rat intestinal cells
Creators Name:	Vandewalle, A., Cluzeaud, F., Peng, K.C., Bens, M., Luechow, A., Guenther, W. and Jentsch, T.J.
Abstract:	ClC-5 is the Cl- channel that is mutated in Dent's disease, an X-chromosome-linked disease characterized by low molecular weight proteinuria, hypercalciuria, and kidney stones. It is predominantly expressed in endocytically active renal proximal cells. We investigated whether this Cl- channel could also be expressed in intestinal tissues that have endocytotic machinery. ClC-5 mRNA was detected in the rat duodenum, jejunum, ileum, and colon. Western blot analyses revealed the presence of the 83-kDa ClC-5 protein in these tissues. Indirect immunofluorescence studies showed that ClC-5 was mainly concentrated in the cytoplasm above the nuclei of enterocytes and colon cells. ClC-5 partially colocalized with the transcytosed polymeric immunoglobulin receptor but was not detectable together with the brush-border-anchored sucrase isomaltase. A subfractionation of vesicles obtained by differential centrifugation showed that ClC-5 is associated with the vacuolar 70-kDa H+-ATPase and the small GTPases rab4 and rab5a, two markers of early endosomes. Thus these results indicate that ClC-5 is present in the small intestine and colon of rats and suggest that it plays a role in the endocytotic pathways of intestinal cells.
Keywords:	Intestine, Endocytosis, H+-ATPase, Rab, Animals, Rats
Source:	American Journal of Physiology Cell Physiology
ISSN:	0363-6143
Publisher:	American Physiological Society
Volume:	280
Number:	2
Page Range:	C373-C381
Date:	February 2001
Official Publication:	http://ajpcell.physiology.org/cgi/content/abstract/280/2/C373
PubMed:	View item in PubMed

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