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Iron absorption and distribution in TNF(DeltaARE/+) mice, a model of chronic inflammation

Item Type:Article
Title:Iron absorption and distribution in TNF(DeltaARE/+) mice, a model of chronic inflammation
Creators Name:Schuemann, K., Herbach, N., Kerling, C., Seifert, M., Fillebeen, C., Prysch, I., Reich, J., Weiss, G. and Pantopoulos, K.
Abstract:Hemizygous TNF(DeltaARE/+) mice are a murine model for chronic inflammation. We utilized these animals to study iron-kinetics and corresponding protein expression in an iron-deficient and iron-adequate setting. (59)Fe-absorption was determined in ligated duodenal loops in vivo. Whole body distribution of i.v. injected (59)Fe was analysed, and the organ specific expression of ferroportin, transferrin receptor-1, hepcidin and duodenal DMT-1 was quantified by real-time PCR and Western blotting. Duodenal (59)Fe-lumen-to-body transport was not affected by the genotype. Duodenal (59)Fe-retention was increased in TNF(DeltaARE/+) mice, suggesting higher (59)Fe-losses with defoliated enterocytes. Iron-deficiency increased duodenal (59)Fe-lumen-to-body transport, and higher duodenal (59)Fe-tissue retention went along with higher duodenal DMT-1, ferroportin, and liver hepcidin expression. TNF(DeltaARE/+) mice significantly increase their (59)Fe-content in inflamed joints and ilea, and correspondingly reduce splenic (59)Fe-content. Leukocyte infiltrations in the joints suggest a substantial shift of iron-loaded RES cells to inflamed tissues as the underlying mechanism. This finding was paralleled by increased non-haem iron content in joints and reduced haemoglobin and haematocrit concentrations in TNF(DeltaARE/+) mice. In conclusion, erythropoiesis in inflamed TNF(DeltaARE/+) mice could be iron-limited due to losses with exfoliated iron-loaded enterocytes and/or to increased iron-retention in RES cells that shift from the spleen to inflamed tissues.
Keywords:Inflammation, Iron-Absorption, Iron-Distribution, Proteins of Iron Homoeostasis, Animals, Mice
Source:Journal of Trace Elements in Medicine and Biology
Page Range:58-66
Date:January 2010
Official Publication:https://doi.org/10.1016/j.jtemb.2009.10.002
PubMed:View item in PubMed

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