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| Item Type: | Article |
|---|---|
| Title: | Targets of the Tal1 transcription factor in erythrocytes: E2 ubiquitin conjugase regulation by Tal1 |
| Creators Name: | Lausen, J., Pless, O., Leonard, F., Kuvardina-Bolling, O.N., Koch, B. and Leutz, A. |
| Abstract: | The Tal1 transcription factor is essential for the development of the hematopoietic system and plays a role during definitive erythropoiesis in the adult. Despite the importance of Tal1 in erythropoiesis only a small number of erythroid differentiation target genes are known. A chromatin precipitation and cloning approach was established to uncover novel Tal1 target genes in erythropoiesis. The BirA-tag/BirA-ligase biotinylation system in combination with streptavidine chromatin precipitation (Strep-CP) was used to co-precipitate genomic DNA bound to Tal1. Tal1 was found to bind in the vicinity of 31 genes including the E2-ubiquitin conjugase UBE2H gene. Binding of Tal1 to UBE2H was confirmed by chromatin immuno-precipitation. UBE2H expression is increased during erythroid differentiation of hCD34+ cells. Tal1 expression activated UBE2H expression whereas Tal1 knock-down reduced UBE2H expression and ubiquitin transfer activity. This study identifies parts of the ubiquitinylation machinery as a cellular target down-stream of the transcription factor Tal1 and provides novel insights into Tal1 regulated erythropoiesis. |
| Keywords: | Blood Erythropoeisis, Chromatin/Immunoprecipitation/ChIP, Development Differentiation/Hematopoietic, Diseases/Cancer/Leukemia, Gene/Transcription, Proteases/Ubiquitination, Transcription |
| Source: | Journal of Biological Chemistry |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Volume: | 285 |
| Number: | 8 |
| Page Range: | 5338-5346 |
| Date: | 19 February 2010 |
| Official Publication: | https://doi.org/10.1074/jbc.M109.030296 |
| PubMed: | View item in PubMed |
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