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Brain-derived neurotrophic factor reduces amyloidogenic processing through control of SORLA gene expression

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Item Type:Article
Title:Brain-derived neurotrophic factor reduces amyloidogenic processing through control of SORLA gene expression
Creators Name:Rohe, M., Synowitz, M., Glass, R., Paul, S.M., Nykjaer, A. and Willnow, T.E.
Abstract:Sorting protein-related receptor with A-type repeats (SORLA) is a major risk factor in cellular processes leading to Alzheimer's disease (AD). It acts as sorting receptor for the amyloid precursor protein (APP) that regulates intracellular trafficking and processing into amyloidogenic-beta peptides (Abeta). Overexpression of SORLA in neurons reduces while inactivation of gene expression (as in knock-out mouse models) accelerates amyloidogenic processing and senile plaque formation. The current study aimed at identifying molecular pathways that control SORLA gene transcription in vivo and that may contribute to low levels of receptor expression in the brain of patients with AD. Using screening approaches in primary neurons, we identified brain-derived neurotrophic factor (BDNF) as a major inducer of Sorla that activates receptor gene transcription through the ERK (extracellular regulated kinase) pathway. In line with a physiological role as regulator of Sorla, expression of the receptor is significantly impaired in mouse models with genetic (Bdnf(-/-)) or disease-related loss of BDNF activity in the brain (Huntington's disease). Intriguingly, exogenous application of BDNF reduced Abeta production in primary neurons and in the brain of wild-type mice in vivo, but not in animals genetically deficient for Sorla. These findings demonstrate that the beneficial effects ascribed to BDNF in APP metabolism act through induction of Sorla that encodes a negative regulator of neuronal APP processing.
Keywords:Amyloid Precursor Protein Secretases, Amyloid beta-Protein, Amyloid beta-Protein Precursor, Brain, Brain-Derived Neurotrophic Factor, Cultured Cells, Cerebral Cortex, Animal Disease Models, Extracellular Signal-Regulated MAP Kinases, Gene Expression Regulation, Huntington Disease, MAP Kinase Signaling System, Membrane Transport Proteins, Neurons, Messenger RNA, trkB Receptor, LDL Receptors, Animals, Mice
Source:Journal of Neuroscience
Publisher:Society for Neuroscience
Page Range:15472-15478
Date:9 December 2009
Additional Information:Copyright (c) 2009 by The Society for Neuroscience
Official Publication:https://doi.org/10.1523/JNEUROSCI.3960-09.2009
PubMed:View item in PubMed

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