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Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression

Item Type:Article
Title:Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression
Creators Name:Stoeckius, M., Maaskola, J., Colombo, T., Rahn, H.P., Friedlaender, M.R., Li, N., Chen, W., Piano, F. and Rajewsky, N.
Abstract:Caenorhabditis elegans is one of the most prominent model systems for embryogenesis, but collecting many precisely staged embryos has been impractical. Thus, early C. elegans embryogenesis has not been amenable to most high-throughput genomics or biochemistry assays. To overcome this problem, we devised a method to collect staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). In a proof-of-principle experiment, we found that a single eFACS run routinely yielded tens of thousands of almost perfectly staged 1-cell stage embryos. As the earliest embryonic events are driven by posttranscriptional regulation, we combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We discovered complex and orchestrated changes in the expression between and within almost all classes of small RNAs, including microRNAs and 26G-RNAs, during embryogenesis.
Keywords:Nonmammalian Embryo, Flow Cytometry, Small Interfering RNA, Animals, Caenorhabditis Elegans
Source:Nature Methods
Publisher:Nature Publishing Group
Page Range:745-751
Date:October 2009
Official Publication:https://doi.org/10.1038/nmeth.1370
PubMed:View item in PubMed

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