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Identification of proteins resembling G-protein alpha subunits in locust muscle

Item Type:Article
Title:Identification of proteins resembling G-protein alpha subunits in locust muscle
Creators Name:Soeling, A., Walther, C. and Rosenthal, W.
Abstract:In locust skeletal muscle, FMRFamide-like peptides decrease a K+ conductance. Functional data suggest the involvement of G-proteins. For identification of G-protein alpha-subunits, membranes of locust skeletal muscle were probed with ADP-ribosylating bacterial toxins, the photoreactive GTP analog, [alpha-32P]GTP azidoanilide, and with antibodies against mammalian alpha-subunits. Multiple guanine nucleotide-binding proteins of approximately 24-95 kDa were detected. Pertussis toxin catalyzed the ADP-ribosylation of two proteins comigrating with the ADP-ribosylated alpha-subunits of the mammalian G-proteins Go and Gi. Cholera toxin promoted ADP-ribosylation of a protein comigrating with mammalian cholera toxin substrates (i.e., Gs alpha-subunits). An antibody against mammalian Go alpha-subunits detected a 54-kDa protein. Thus proteins with properties of mammalian G-protein subunits are present in insect muscle.
Keywords:Adenosine Triphosphate, Affinity Labels, Azides, GTP-Binding Proteins, Gene Library, Guanosine Triphosphate, Immunoblotting, Macromolecular Substances, Molecular Weight, Muscles, Pertussis Toxin, Bordetella Virulence Factors, Animals, Grasshoppers
Source:Biochemical and Biophysical Research Communications
Publisher:Academic Press
Page Range:1075-1082
Date:31 October 1991
Official Publication:https://doi.org/10.1016/S0006-291X(05)81176-3
PubMed:View item in PubMed

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