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| Item Type: | Article |
|---|---|
| Title: | Identification of proteins resembling G-protein alpha subunits in locust muscle |
| Creators Name: | Soeling, A., Walther, C. and Rosenthal, W. |
| Abstract: | In locust skeletal muscle, FMRFamide-like peptides decrease a K+ conductance. Functional data suggest the involvement of G-proteins. For identification of G-protein alpha-subunits, membranes of locust skeletal muscle were probed with ADP-ribosylating bacterial toxins, the photoreactive GTP analog, [alpha-32P]GTP azidoanilide, and with antibodies against mammalian alpha-subunits. Multiple guanine nucleotide-binding proteins of approximately 24-95 kDa were detected. Pertussis toxin catalyzed the ADP-ribosylation of two proteins comigrating with the ADP-ribosylated alpha-subunits of the mammalian G-proteins Go and Gi. Cholera toxin promoted ADP-ribosylation of a protein comigrating with mammalian cholera toxin substrates (i.e., Gs alpha-subunits). An antibody against mammalian Go alpha-subunits detected a 54-kDa protein. Thus proteins with properties of mammalian G-protein subunits are present in insect muscle. |
| Keywords: | Adenosine Triphosphate, Affinity Labels, Azides, GTP-Binding Proteins, Gene Library, Guanosine Triphosphate, Immunoblotting, Macromolecular Substances, Molecular Weight, Muscles, Pertussis Toxin, Bordetella Virulence Factors, Animals, Grasshoppers |
| Source: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| Publisher: | Academic Press |
| Volume: | 180 |
| Number: | 2 |
| Page Range: | 1075-1082 |
| Date: | 31 October 1991 |
| Official Publication: | https://doi.org/10.1016/S0006-291X(05)81176-3 |
| PubMed: | View item in PubMed |
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