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Transcriptional profiling of CD31(+) cells isolated from murine embryonic stem cells

Item Type:Article
Title:Transcriptional profiling of CD31(+) cells isolated from murine embryonic stem cells
Creators Name:Mariappan, D., Winkler, J., Chen, S., Schulz, H., Hescheler, J. and Sachinidis, A.
Abstract:Identification of genes involved in endothelial differentiation is of great interest for the understanding of the cellular and molecular mechanisms involved in the development of new blood vessels. Mouse embryonic stem (mES) cells serve as a potential source of endothelial cells for transcriptomic analysis. We isolated endothelial cells from 8-days old embryoid bodies by immuno-magnetic separation using platelet endothelial cell adhesion molecule-1 (also known as CD31) expressed on both early and mature endothelial cells. CD31(+) cells exhibit endothelial-like behavior by being able to incorporate DiI-labeled acetylated low-density lipoprotein as well as form tubular structures on matrigel. Quantitative and semi-quantitative PCR analysis further demonstrated the increased expression of endothelial transcripts. To ascertain the specific transcriptomic identity of the CD31(+) cells, large-scale microarray analysis was carried out. Comparative bioinformatic analysis reveals an enrichment of the gene ontology categories angiogenesis, blood vessel morphogenesis, vasculogenesis and blood coagulation in the CD31(+) cell population. Based on the transcriptomic signatures of the CD31(+) cells, we conclude that this ES cell-derived population contains endothelial-like cells expressing a mesodermal marker BMP2 and possess an angiogenic potential. The transcriptomic characterization of CD31(+) cells enables an in vitro functional genomic model to identify genes required for angiogenesis.
Keywords:CD31 Antigens, Biological Markers, Body Patterning, Cell Differentiation, Cultured Cells, Embryonic Stem Cells, Endothelial Cells, Gene Expression Profiling, Developmental Gene Expression Regulation, Germ Layers, Heart, Myocardium, Smooth Muscle Myocytes, Oligonucleotide Array Sequence Analysis, Organ Specificity, Organogenesis, Signal Transduction, Animals, Mice
Source:Genes to Cells
Page Range:243-260
Date:February 2009
Official Publication:https://doi.org/10.1111/j.1365-2443.2008.01268.x
PubMed:View item in PubMed

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