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Cell-penetrating HIV1 TAT peptides float on model lipid bilayers

Item Type:Article
Title:Cell-penetrating HIV1 TAT peptides float on model lipid bilayers
Creators Name:Ciobanasu, C., Harms, E., Tünnemann, G., Cardoso, M.C. and Kubitscheck, U.
Abstract:Cell penetrating peptides like the HIV1 TAT peptide are able to translocate across cell membranes and to carry molecular cargoes into the cellular interior. For most of these peptides the biophysical mechanism of the membrane translocation is still quite unknown. We analyzed HIV1 TAT peptide binding and migration within biological model membranes. To this end we generated giant unilamellar vesicles (GUVs), and characterized the mobility of fluorescently labeled lipids within liquid-ordered and liquid-disordered lipid phases by single molecule tracking. Fluorescently labeled TAT peptides were efficiently binding to anionic GUVs, and less marked to neutral GUVs. Single molecule tracking revealed that HIV1 TAT peptides move on the GUV surface faster than fluorescent lipid analoga and independent of the phase state of the membrane. We concluded that the TAT peptides were not aggregated and not incorporated into but rather floating on the membrane.
Keywords:Anions, Cations, Fluorescent Dyes, tat Gene Products, HIV-1, Hydrophobicity, Lipid Bilayers, Biological Models, Peptides, Permeability, Phosphatidylethanolamines, Protein Transport, Static Electricity, Unilamellar Liposomes, Viscosity
Publisher:American Chemical Society
Page Range:4728-4737
Date:9 June 2009
Official Publication:https://doi.org/10.1021/bi900365s
PubMed:View item in PubMed

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