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Active promoters and insulators are marked by the centrosomal protein 190

Item Type:Article
Title:Active promoters and insulators are marked by the centrosomal protein 190
Creators Name:Bartkuhn, M., Straub, T., Herold, M., Herrmann, M., Rathke, C., Saumweber, H., Gilfillan, G.D., Becker, P.B. and Renkawitz, R.
Abstract:For the compact Drosophila genome, several factors mediating insulator function, such as su(Hw) and dCTCF, have been identified. Recent analyses showed that both these insulator-binding factors are functionally dependent on the same cofactor, CP190. Here we analysed genome-wide binding of CP190 and dCTCF. CP190 binding was detected at CTCF, su(Hw) and GAF sites and unexpectedly at the transcriptional start sites of actively transcribed genes. Both insulator and transcription start site CP190-binding elements are strictly marked by a depletion of histone H3 and, therefore, a loss of nucleosome occupancy. In addition, CP190/dCTCF double occupancy was seen at the borders of many H3K27me3 'islands'. As before, these sites were also depleted of H3. Loss of either dCTCF or CP190 causes an increase of H3 and H3K27 trimethylation at these sites. Thus, for both types of cis-regulatory elements, domain borders and promoters, the chromatin structure is dependent on CP190.
Keywords:Chromatin Domains, Chromatin Insulation, CP190, CTCF, Transcription, Animals, Drosophila-Melanogaster
Source:EMBO Journal
Publisher:Nature Publishing Group
Page Range:877-888
Date:8 April 2009
Official Publication:https://doi.org/10.1038/emboj.2009.34
PubMed:View item in PubMed

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