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| Item Type: | Article |
|---|---|
| Title: | Identification of Rab3-, Rab5a- and synaptobrevin II-like proteins in a preparation of rat kidney vesicles containing the vasopressin-regulated water channel |
| Creators Name: | Liebenhoff, U. and Rosenthal, W. |
| Abstract: | According to the 'shuttle hypothesis', vasopressin increases the water permeability of renal epithelial cells by exocytotic fusion of vesicles containing the water channel AQP-CD with the apical plasma membrane, whereas withdrawal of vasopressin results in endocytotic uptake of AQP-CD. The proteins involved in the redistribution of AQP-CD have not been identified. With a panel of monoclonal antibodies, we detected Rab3-, Rab5a- and synaptobrevin II-like proteins in a kidney preparation enriched in AQP-CD-containing vesicles. The synaptobrevin II-like proteins is not identical with the ubiquitous cellubrevin. Rab3- and synaptobrevin II- but not Rab5a-like proteins were co-enriched with AQP-CD. The data suggest that the proteins involved in hormonal regulation of water permeability in kidney epithelial cells are identical or similar to those involved in regulated exocytosis in secretory cells. |
| Keywords: | Antidiuresis, Water Channel, AQP-CD, Exocytosis, Small GTP-binding Protein, Synaptobrevin, Animals, Rats |
| Source: | FEBS Letters |
| ISSN: | 0014-5793 |
| Publisher: | Elsevier |
| Volume: | 365 |
| Number: | 2-3 |
| Page Range: | 209-213 |
| Date: | 29 May 1995 |
| Official Publication: | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-3YRNY2V-BN&_user=100084&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000007518&_version=1&_urlVersion=0&_userid=100084&md5=62696b17b2d0b48220223a70a4d244c4 |
| PubMed: | View item in PubMed |
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