Identification of Rab3-, Rab5a- and synaptobrevin II-like proteins in a preparation of rat kidney vesicles containing the vasopressin-regulated water channel

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Item Type:	Article
Title:	Identification of Rab3-, Rab5a- and synaptobrevin II-like proteins in a preparation of rat kidney vesicles containing the vasopressin-regulated water channel
Creators Name:	Liebenhoff, U. and Rosenthal, W.
Abstract:	According to the 'shuttle hypothesis', vasopressin increases the water permeability of renal epithelial cells by exocytotic fusion of vesicles containing the water channel AQP-CD with the apical plasma membrane, whereas withdrawal of vasopressin results in endocytotic uptake of AQP-CD. The proteins involved in the redistribution of AQP-CD have not been identified. With a panel of monoclonal antibodies, we detected Rab3-, Rab5a- and synaptobrevin II-like proteins in a kidney preparation enriched in AQP-CD-containing vesicles. The synaptobrevin II-like proteins is not identical with the ubiquitous cellubrevin. Rab3- and synaptobrevin II- but not Rab5a-like proteins were co-enriched with AQP-CD. The data suggest that the proteins involved in hormonal regulation of water permeability in kidney epithelial cells are identical or similar to those involved in regulated exocytosis in secretory cells.
Keywords:	Antidiuresis, Water Channel, AQP-CD, Exocytosis, Small GTP-binding Protein, Synaptobrevin, Animals, Rats
Source:	FEBS Letters
ISSN:	0014-5793
Publisher:	Elsevier
Volume:	365
Number:	2-3
Page Range:	209-213
Date:	29 May 1995
Official Publication:	http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-3YRNY2V-BN&_user=100084&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000007518&_version=1&_urlVersion=0&_userid=100084&md5=62696b17b2d0b48220223a70a4d244c4
PubMed:	View item in PubMed

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