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| Item Type: | Article |
|---|---|
| Title: | Folding and cell surface expression of the vasopressin V2 receptor: requirement of the intracellular C-terminus |
| Creators Name: | Oksche, A., Dehe, M., Schuelein, R., Wiesner, B. and Rosenthal, W. |
| Abstract: | We characterized truncations of the human vasopressin V2 receptor to determine the role of the intracellular C-terminus (comprising about 44 amino acids) in receptor function and cell surface expression. In contrast to the wild-type receptor, the naturally occurring mutant R337X failed to confer specific [3H]AVP binding to transfected cells. In addition, no vasopressin-sensitive adenylyl cyclase was detectable in membrane preparations of these cells. Laser scanning microscopy revealed that c-myc epitope- or green fluorescent protein-tagged R337X mutant receptors were retained within the endoplasmic reticulum. Increasing the number of C-terminal residues (truncations after codons 348, 354 and 356) restored G protein coupling, but revealed a length-dependent reduction of cell surface expression. Replacement of positively charged residues within the C-terminus by glutamine residues also decreased cell surface expression. A chimeric V2 receptor with the C-terminus replaced by that of the beta2-adrenergic receptor did not bind [3H]AVP and was retained within the cell. These data suggest that residues in the N-terminal part of the C-terminus are necessary for correct folding and that C-terminal residues are important for efficient cell surface expression. |
| Keywords: | Surface Expression, Transport, G Protein-Coupled Receptor, Diabetes Insipidus, Animals, Dogs |
| Source: | FEBS Letters |
| ISSN: | 0014-5793 |
| Publisher: | Elsevier |
| Volume: | 424 |
| Number: | 1-2 |
| Page Range: | 57-62 |
| Date: | 6 March 1998 |
| Official Publication: | https://doi.org/10.1016/S0014-5793(98)00140-9 |
| PubMed: | View item in PubMed |
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