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| Item Type: | Article |
|---|---|
| Title: | Ht31: the first protein kinase A anchoring protein to integrate protein kinase A and Rho signaling |
| Creators Name: | Klussmann, E., Edemir, B., Pepperle, B., Tamma, G., Henn, V., Klauschenz, E., Hundsrucker, C., Maric, K. and Rosenthal, W. |
| Abstract: | In an attempt to isolate protein kinase A anchoring proteins (AKAPs) involved in vasopressin-mediated water reabsorbtion, the complete sequence of the human AKAP Ht31 was determined and a partial cDNA of its rat orthologue (Rt31) was cloned. The Ht31 cDNA includes the estrogen receptor cofactor Brx and the RhoA GDP/GTP exchange factor proto-lymphoid blast crisis (Lbc) sequences. The Ht31 gene was assigned to chromosome 15 (region q24-q25). It encodes Ht31 and the smaller splice variants Brx and proto-Lbc. A protein of the predicted size of Ht31 (309 kDa) was detected in human mammary carcinoma and HeLa cells. Anti-Ht31/Rt31 antibodies immunoprecipitated RhoA from primary cultured rat renal inner medullary collecting duct cells, indicating an interaction between the AKAP and RhoA in vivo. These results suggest that Ht31/Rt31 represent a new type of AKAP, containing both an anchoring and a catalytic domain, which appears to be capable of modulating the activity of an interacting partner. Ht31/Rt31 have the potential to integrate Rho and protein kinase A signaling pathways, and thus, are prime candidates to regulate vasopressin-mediated water reabsorbtion. |
| Keywords: | Protein kinase A anchoring protein, Rho, Rho GTPase guanine nucleotide exchange factor, Ht31, Brx, Aquaporin, Animals, Rats |
| Source: | FEBS Letters |
| ISSN: | 0014-5793 |
| Publisher: | Elsevier |
| Volume: | 507 |
| Number: | 3 |
| Page Range: | 264-268 |
| Date: | 2 November 2001 |
| Official Publication: | https://doi.org/10.1016/S0014-5793(01)02995-7 |
| PubMed: | View item in PubMed |
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