Functional involvement of VAMP/synaptobrevin-2 in cAMP-stimulated aquaporin 2 translocation in renal collecting duct cells

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Item Type:	Article
Title:	Functional involvement of VAMP/synaptobrevin-2 in cAMP-stimulated aquaporin 2 translocation in renal collecting duct cells
Creators Name:	Gouraud, S., Laera, A., Calamita, G., Carmosino, M., Procino, G., Rossetto, O., Mannucci, R., Rosenthal, W., Svelto, M. and Valenti, G.
Abstract:	The involvement of soluble N-ethylmaleimide sensitive factor-attachment protein receptor (SNARE) proteins in the cAMP-induced exocytosis of aquaporin 2 (AQP2)-containing vesicles was investigated in AQP2-transfected renal CD8 cells. RT-PCR and western blot analysis confirmed the presence of the SNARE homologs VAMP/synaptobrevin-2, syntaxin-1, syntaxin-4 and SNAP-23 in CD8 cells. Tetanus neurotoxin (TeNT) was efficient in cleaving synaptobrevin-like protein both in vitro and in intact CD8 cells incubated with the toxin. TeNT treatment in intact CD8 cells completely abolished cAMP-stimulated AQP2 targeting to the plasma membrane, as assessed by quantification of cell-surface immunoreactivity to an anti-AQP2 antibody raised against a peptide reproducing the extracellular AQP2 C-loop. These results represent the first evidence for the functional involvement of VAMP-2 in cAMP-induced AQP2 exocytosis in renal cells.
Keywords:	Aquaporin, SNARE, Tetanus Toxin, Trafficking, Exocytosis, Animals, Rabbits
Source:	Journal of Cell Science
ISSN:	0021-9533
Publisher:	Company of Biologists
Volume:	115
Number:	Pt 18
Page Range:	3667-3674
Date:	15 September 2002
Official Publication:	https://doi.org/10.1242/jcs.00053
PubMed:	View item in PubMed

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