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Distinct and overlapping roles of CXCR5 and CCR7 in B-1 cell homing and early immunity against bacterial pathogens

Item Type:Article
Title:Distinct and overlapping roles of CXCR5 and CCR7 in B-1 cell homing and early immunity against bacterial pathogens
Creators Name:Höpken, U.E. and Achtman, A.H. and Krueger, K. and Lipp, M.
Abstract:CXC chemokine receptor (CXCR)5 and CC chemokine receptor (CCR)7 are the major chemokine receptors required for B cell homing and microenvironmental localization during antigen-independent and -dependent B cell differentiation. Here, we show markedly decreased B-1 B cell numbers in the peritoneal cavity of CXCR5 -/- and CXCR5 -/-CCR7 -/- double-deficient mice paralleled by reduced antigen-induced phosphorylcholine-specific immunoglobulin (Ig)M responses after intraperitoneal (i.p.) administration of streptococcal antigen. CCR7 -/- mice also revealed a partial reduction in peritoneal B-1 cell numbers combined with a reduced humoral response to i.p. injected bacterial antigen. However, opposite roles of CXCR5 and CCR7 were observed when the frequency of peritoneal B-2 cells was analyzed. CXCR5 -/- mice almost completely lacked B-2 cells, whereas CCR7 deficiency engendered an increase in peritoneal B-2 cells. In addition, CCR7 -/- mice had enhanced, splenic IgM + plasma cell responses, whereas the extrafollicular B cell response of the CXCR5 -/- mice was not significantly altered compared with wild-type controls. Thus, the two chemokine receptors exert divergent forces at multiple levels of the innate immune response. CXCR5 plays a dominant role in peritoneal B-1 B cell homing and body cavity immunity, but both chemokine receptors are needed for a proportional peritoneal B-2 cell homing and balanced development of an early splenic B cell response.
Keywords:B Cells, Body Cavity Immunity, Chemokine Receptor, Immunoglobulin M, Animals, Mice
Source:Journal of Leukocyte Biology
Publisher:Federation of American Societies for Experimental Biology
Page Range:709-718
Date:September 2004
Official Publication:https://doi.org/10.1189/jlb.1203643
PubMed:View item in PubMed

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