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A Drosophila homolog of the yeast origin recognition complex

Item Type:Article
Title:A Drosophila homolog of the yeast origin recognition complex
Creators Name:Gossen, M. and Pak, D.T. and Hansen, S.K. and Acharya, J.K. and Botchan, M.R.
Abstract:Genes from Drosophila melanogaster have been identified that encode proteins homologous to Orc2p and Orc5p of the Saccharomyces cerevisiae origin recognition complex (ORC). The abundance of the Drosophila Orc2p homolog DmORC2 is developmentally regulated and is greatest during the earliest stages of embryogenesis, concomitant with the highest rate of DNA replication. Fractionation of embryo nuclear extracts revealed that DmORC2 is found in a tightly associated complex with five additional polypeptides, much like the yeast ORC. These studies will enable direct testing of the initiator-based model of replication in a metazoan.
Keywords:Amino Acid Sequence, Molecular Cloning, DNA Replication, Complementary DNA, DNA-Binding Proteins, Nonmammalian Embryo, Insect Genes, Molecular Sequence Data, Molecular Weight, Origin Recognition Complex, Replication Origin, Repressor Proteins, Saccharomyces Cerevisiae, Sequence Homology, Animals, Drosophila Melanogaster
Publisher:American Association for the Advancement of Science
Page Range:1674-1677
Date:8 December 1995
Official Publication:https://doi.org/10.1126/science.270.5242.1674
PubMed:View item in PubMed

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