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Efficient gene transfer into primary human CD8+ T lymphocytes by MuLV-10A1 retrovirus pseudotype

Item Type:Article
Title:Efficient gene transfer into primary human CD8+ T lymphocytes by MuLV-10A1 retrovirus pseudotype
Creators Name:Uckert, W. and Becker, C. and Gladow, M. and Klein, D. and Kammertoens, T. and Pedersen, L. and Blankenstein, T.
Abstract:Efficient and stable gene transfer into primary human T lymphocytes would greatly improve their use for adoptive transfer to treat acquired disorders, viral diseases, and cancer. We have constructed retroviral vector pseudotypes of amphotropic murine leukemia viruses (A-MuLV, MuLV-10A1), gibbon ape leukemia virus (GaLV), and feline endogenous virus (RD114) containing the enhanced green fluorescent protein (GFP) as a marker gene. Transduction of primary human CD8+ T lymphocytes by the different GFP- retrovirus pseudotypes revealed the superiority of MuLV-10A1 in comparison with A-MuLV, GaLV, and RD114, respectively. The superior transduction efficacy of CD8+ T cells by MuLV-10A1 correlates with a longer half-life of this pseudotype in comparison with A-MuLV and, as shown by interference analysis with the human T cell line HUT78, by the utilization of both the A- MuLV receptor (Pit2) and the GaLV receptor (Pit1) for cell entry.
Keywords:CD8-Positive T-Lymphocytes, Gene Transfer Techniques, Genetic Vectors, Genetic Transduction, Green Fluorescent Proteins, Luminescent Proteins Leukemia Virus, Murine, Retroviridae, Virus Receptors
Source:Human Gene Therapy
Publisher:Mary Ann Liebert
Page Range:1005-1014
Date:1 January 2000
Official Publication:https://doi.org/10.1089/10430340050015310
PubMed:View item in PubMed

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