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| Item Type: | Preprint |
|---|---|
| Title: | Supporting-like cells constitute an alternative steroidogenic lineage conserved in amniotes |
| Creators: |
Barberá-Aura, Iván  ORCID: https://orcid.org/0009-0007-6058-3796, Chung, Wai-Yee, Dujardin, Emilie ORCID: https://orcid.org/0000-0002-8189-8742, Hurtado, Alicia ORCID: https://orcid.org/0000-0001-6267-704X, Galmiche, Mathieu ORCID: https://orcid.org/0000-0001-5840-4786, Mayère, Chloé ORCID: https://orcid.org/0000-0002-9254-4886, Guy, Maëva ORCID: https://orcid.org/0009-0007-7802-5186, Mandon-Pépin, Béatrice ORCID: https://orcid.org/0000-0002-5424-0822, Franke, Vedran ORCID: https://orcid.org/0000-0003-3606-6792, Jaszczyszyn, Yan ORCID: https://orcid.org/0000-0002-2269-677X, Acemel, Rafael D. ORCID: https://orcid.org/0000-0003-4120-0140, Rudaz, Serge ORCID: https://orcid.org/0000-0002-4180-5417, Mckey, Jennifer ORCID: https://orcid.org/0000-0002-2640-1502, Nef, Serge ORCID: https://orcid.org/0000-0001-5462-0676, Pailhoux, Eric ORCID: https://orcid.org/0000-0001-8012-9143 and Lupiáñez, Darío G. ORCID: https://orcid.org/0000-0002-3165-036X
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| Abstract: | In mammals, the production of sex hormones is widely considered to depend on the interstitial lineage of the gonad, which differentiates into Leydig cells in males or theca cells in females. However, certain mammalian species evidence gonadal steroidogenic activity prior to the specialization of these interstitial lineages, suggesting that alternative cell types may assume this function. Here we reveal a previously unrecognized role for supporting-like cells (SLCs), which can act as a major steroidogenic lineage during mammalian embryonic development. Through comparative single-cell transcriptomics, steroidomics and in toto organ imaging we find that in rabbits, SLCs not only contribute to the formation of gonadal rete structures, as described for other mammals, but also differentiate into a steroid-producing population. The steroidogenic program of SLCs is initially activated in both sexes but selectively maintained in ovaries, whereas in testes it is progressively replaced by that of interstitially derived Leydig cells. Evolutionary comparisons indicate that SLCs may represent an ancestral lineage that is homologous to the steroidogenic cells of non-mammalian species, which also derive from supporting precursors and share expression of cell fate regulators such as PAX2/8 and TBX1. Altogether, our findings redefine current models of gonadal lineages, revealing an unexpected plasticity in sex differentiation and exemplifying how distinct cell types can converge on analogous functions during evolution. |
| Keywords: | Animals, Rabbits |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2026.05.25.726204 |
| Date: | 28 May 2026 |
| Official Publication: | https://doi.org/10.64898/2026.05.25.726204 |
| Related to: |
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