Single-cell mitochondrial genotypoing of exonuclease-deficient DNA polymerase γ (POLGD274A) knock-in HEK293 cell lines

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Item Type:	Dataset
Title:	Single-cell mitochondrial genotypoing of exonuclease-deficient DNA polymerase γ (POLGD274A) knock-in HEK293 cell lines
Creators Name:	Hsieh, Y.H., Liebold, J. and Ludwig, L.S.
Abstract:	Exonuclease-deficient DNA polymerase γ (POLGD274A) lead to an elevated mutational rate and hypermutated mitochondrial genomes. Here, leverage mitochondrial single-cell ATAC-seq (mtscATAC-seq) to estimate genome-wide mtDNA mutational burden in HEK293 cell lines with POLGD274A knock-in. Our method sensitively detects up to a a median of 472 variants per cell, corresponding to a 50-fold increase comared to control, with a predominance of replication-driven C>T transitions.
Source:	Gene Expression Omnibus (GEO)
Publisher:	National Center for Biotechnology Information (NCBI)
Date:	31 December 2026
Additional Information:	Private data, not to be shared or distributed without permission.
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Related to:	URL URL Type https://edoc.mdc-berlin.de/26049/ Publication

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