![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
5MB |
![[thumbnail of Supplemental Information]](https://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplemental Information)
22MB |
| Item Type: | Article |
|---|---|
| Title: | Alternative splicing dynamics during human cardiac development in vivo and in vitro |
| Creators: |
Gomes-Silva, Beatriz, Furtado, Marta  ORCID: https://orcid.org/0000-0003-3714-9653, Ribeiro, Marta, Martins, Sandra, Carvalho, Teresa, Ventura-Gomes, André, Maatz, Henrike ORCID: https://orcid.org/0000-0002-9232-6272, Parakkat, Pragati ORCID: https://orcid.org/0000-0003-2691-791X, Crocini, Claudia, Gotthardt, Michael ORCID: https://orcid.org/0000-0003-1788-3172, Savisaar, Rosina and Carmo-Fonseca, Maria ORCID: https://orcid.org/0000-0002-3402-7143
|
| Abstract: | Cardiomyocytes differentiated in vitro from human induced pluripotent stem cells (iPSC-CMs) are increasingly used in studies of disease mechanisms, drug development, toxicity testing, and regenerative medicine. Alternative splicing (AS) plays a pivotal role in cardiac development. However, the extent to which iPSC-CMs recapitulate native cardiac splicing patterns remains poorly understood. Here, we provide a comprehensive temporal map of AS regulation during human cardiac development. iPSC-derived cardiomyocytes globally recapitulate the transcriptome of prenatal cardiomyocytes, yet their splicing profiles remain heterogeneous, with certain events reflecting early embryonic patterns and others resembling those of later-stage fetal hearts. Moreover, we uncover altered splicing events in iPSC-CMs, including mis-splicing of splicing factors. In conclusion, we present a resource of AS dynamics throughout human cardiac development and a catalog of splicing markers to assess cardiomyocyte maturation in vitro. Our findings provide critical insights into the limitations of iPSC-CM models and their utility in cardiovascular research. |
| Keywords: | Cardiac Myocytes, Pluripotent Stem Cells, Cell Differentiation, Developmental Gene Expression Regulation, Heart, Induced Pluripotent Stem Cells, Organogenesis, Transcriptome |
| Source: | Stem Cell Reports |
| ISSN: | 2213-6711 |
| Publisher: | Cell Press / Elsevier |
| Volume: | 21 |
| Number: | 1 |
| Page Range: | 102757 |
| Date: | 13 January 2026 |
| Official Publication: | https://doi.org/10.1016/j.stemcr.2025.102757 |
| PubMed: | View item in PubMed |
| Related to: |
Repository Staff Only: item control page
