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| Item Type: | Preprint |
|---|---|
| Title: | Interleukin-17A mediates cardiorenal injury in oxalate nephropathy |
| Creators: |
Wimmer, Moritz I.  ORCID: https://orcid.org/0009-0004-5793-0249, Reichel, Martin ORCID: https://orcid.org/0000-0001-7886-2784, Thiele, Arne ORCID: https://orcid.org/0000-0003-0786-2239, Yarritu, Alex ORCID: https://orcid.org/0000-0002-1724-0559, Matz-Rauch, Ariana ORCID: https://orcid.org/0000-0002-5152-5830, Anandakumar, Harithaa ORCID: https://orcid.org/0009-0000-3326-5126, Götz, Luisa Hernandez ORCID: https://orcid.org/0009-0001-0560-1548, Lesker, Till Robin ORCID: https://orcid.org/0000-0002-3085-6438, Potapenko, Olena ORCID: https://orcid.org/0000-0002-4397-568X, Gebremedhin, Natnael ORCID: https://orcid.org/0009-0001-2250-9436, Anders, Wibke ORCID: https://orcid.org/0009-0000-6069-4233, Liévano Contreras, Sarah V. ORCID: https://orcid.org/0009-0000-9967-0108, Wang, Rongling ORCID: https://orcid.org/0000-0001-8719-938X, Nonn, Olivia ORCID: https://orcid.org/0000-0001-6385-2866, Schiattarella, Gabriele G. ORCID: https://orcid.org/0000-0002-7582-7171, Schaefer, Franz ORCID: https://orcid.org/0000-0001-7564-9937, Holle, Johannes ORCID: https://orcid.org/0000-0001-8032-4096, Strowig, Till ORCID: https://orcid.org/0000-0003-0185-1459, Zernecke, Alma ORCID: https://orcid.org/0000-0001-8551-4729, Eckardt, Kai-Uwe ORCID: https://orcid.org/0000-0003-3823-0920, Knauf, Felix ORCID: https://orcid.org/0000-0001-5762-1404, Wilck, Nicola ORCID: https://orcid.org/0000-0003-3189-5364 and Bartolomaeus, Hendrik ORCID: https://orcid.org/0000-0003-4288-3828
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| Abstract: | AIMS: Cardiovascular disease (CVD) is the leading cause of mortality in chronic kidney disease (CKD). While CKD is known to give rise to systemic inflammation, its inciting factors remain poorly defined. Oxalate, long implicated in rare genetic kidney disorders, accumulates with decreased kidney function and has emerged as a driver of inflammation and independent risk factor for CVD. Here, we investigate the immunological mechanisms linking oxalate nephropathy to systemic inflammation, cardiac damage and kidney injury. METHODS AND RESULTS: Oxalate nephropathy was induced in C57Bl6/N mice via an oxalate-enriched diet. Oxalate induced systemic immune activation, renal fibrosis, and cardiac remodeling, including pulmonary congestion with systolic and diastolic dysfunction. Flow cytometry analysis identified interleukin (IL)-17A as a dominant inflammatory effector, with expansion of Th17 and Th17-like Treg in the kidney, intestine, and spleen. Bulk mRNA sequencing confirmed these findings. Confirming the oxalate-IL-17A relationship, plasma IL-17A was elevated in patients with primary hyperoxaluria. Gut microbiome analysis by 16S amplicon sequencing showed only mild oxalate-induced alterations in mice. However, soluble oxalate directly enhanced Th17 polarization and disrupted mitochondrial respiration in vitro. In vivo, antibody-mediated (clone: 17F3) IL-17A blockade decreased cardiac fibrosis, reduced neutrophil infiltration, and partially restored cardiac function in oxalate-fed mice. CONCLUSIONS: Our study identifies oxalate as a systemic immunometabolic stressor and IL-17A as a central mediator of oxalate-induced cardiorenal injury. These findings establish the oxalate–IL 17A axis as a mechanistic link between CKD and CVD and suggest IL-17A inhibition as a potential therapeutic strategy to reduce cardiovascular damage in CKD. |
| Keywords: | Animals, Mice |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2025.11.17.687153 |
| Date: | 18 November 2025 |
| Official Publication: | https://doi.org/10.1101/2025.11.17.687153 |
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