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| Item Type: | Dataset |
|---|---|
| Title: | Gestational diabetes mellitus alters placental pre-mRNA splicing |
| Creators Name: | Engal, Eden, Gershon, Adi, Melamed, Shiri, Sharma, Aveksha, Ner-Gaon, Hadas, Jaffe-Herman, Shiri, Nevo, Yuval, Kirzhner, Alena, Barak, Oren, Vaisbuch, Edi, Kay, Gillian, Staff, Anne Cathrine, Dechend, Ralf, Herse, Florian, Shay, Tal, Salton, Maayan and Schiller, Tal |
| Abstract: | Gestational diabetes mellitus (GDM) is defined as hyperglycemia first identified during pregnancy and can lead to adverse maternal and neonatal outcomes. The molecular mechanisms leading to these outcomes are currently poorly understood. While transcriptomics of GDM placentas has been previously studied, the effect on pre-mRNA splicing remains largely unknown. This study explores the impact of GDM on placental splicing and identifies its regulatory mechanisms. Using RNA-sequencing data from European and Chinese cohorts, we uncovered thousands of differential splicing events. Pathway enrichment analysis revealed significant associations with metabolic and diabetes-related pathways. Splicing factor motif and CLIP-seq analyses highlighted SRSF10 as a key regulator in this process, with its binding enriched at mis-spliced exons. Silencing SRSF10 in placental cells mirrored GDM-associated mis-splicing in key genes. These findings underscore splicing dysregulation as a critical process in GDM pathogenesis, suggesting that targeting SRSF10 could be a potential therapeutic approach to mitigate the deleterious effects of GDM. |
| Keywords: | Diabetes in Pregnancy, Alternative Pre-mRNA Splicing, Placenta, Pregnancy, Pregnancy-Associated Diabetes |
| Source: | figshare |
| Publisher: | Digital Science |
| Date: | 18 November 2025 |
| Official Publication: | https://doi.org/10.2337/figshare.30275872.v1 |
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