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| Item Type: | Preprint |
|---|---|
| Title: | Evolutionarily conserved transcriptional regulators control monoaminergic neuron development |
| Creators: |
Lewis, Clifton  ORCID: https://orcid.org/0000-0002-3077-8150, Goulty, Matthew ORCID: https://orcid.org/0009-0001-5731-6722, Wroblewska, Aniela, Croxall, Nicola ORCID: https://orcid.org/0009-0003-1061-6909, Onion, David ORCID: https://orcid.org/0000-0002-4522-2307, Robinson, Sue, Zinzen, Robert P. ORCID: https://orcid.org/0000-0002-8638-5102, Solana, Jordi ORCID: https://orcid.org/0000-0002-6770-3929, Kyriacou, Charalambos P. ORCID: https://orcid.org/0000-0003-4889-7606, Rosato, Ezio ORCID: https://orcid.org/0000-0001-7297-4697 and Feuda, Roberto ORCID: https://orcid.org/0000-0003-0857-1732
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| Abstract: | To what extent conserved developmental programs specify homologous cell types is a central question in biology. Here, we address this by focusing on reconstructing monoaminergic neuron development in Drosophila melanogaster embryo using time-resolved single-cell genomics, spatial transcript mapping with hybridisation chain reaction, and targeted metabolomics. We uncover a regulatory landscape in which specific transcription factors are activated before biosynthetic enzymes, establishing a prospective temporal architecture for monoaminergic fate specification. Comparative analyses of developmental single-cell atlases from zebrafish and sea urchin indicate that components of this machinery are conserved across ~550 million years of bilaterian evolution with orthologous transcription factors showing similar temporal dynamics. Together, these findings point to a putatively conserved regulatory core that interfaces with other context-dependent transcription factors; this interplay accommodates monoaminergic multifunction and subtype diversity across distinct neuroanatomies. |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2025.10.29.685200v2 |
| Date: | 31 October 2025 |
| Official Publication: | https://doi.org/10.1101/2025.10.29.685200 |
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