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| Item Type: | Preprint |
|---|---|
| Title: | Unraveling the single-cell spatial landscapes of melanoma brain metastases |
| Creators Name: | Manukyan, Artür, Peters, Kristin, Radbruch, Helena, Fusco, Federico, Geserick, Felicitas, Rossner, Florian, Lehmann, Annika, Kleo, Karsten, Conrad, Thomas, Altmüller, Janine, Plumbom, Izabela, Akalin, Altuna, Jürchott, Karsten, Wyler, Emanuel, Landthaler, Markus, Delbridge, Claire, Sendler, Matthias, Radke, Josefine and Redmer, Torben |
| Abstract: | The cellular composition of the tumor ecosystem is a critical determinant of therapeutic responsiveness to immune checkpoint inhibitors (ICi) and BRAF inhibitors (BRAFi). However, the specific compositional dynamics that drive the subsequent progression of melanoma brain metastases (MBMs) remain poorly understood. Here, we performed single cell-resolved spatial transcriptomics profiling of multiple tissue cores (n=47) of distinct solitary and concordant multiple MBM (n=8), CNS tissue (n=7) and whole sections including one matched primary (n=4) of MBMs across various developmental stages and therapeutic regimens. Our approach uncovered both shared and distinct evolutionary patterns of MBM progression, revealing pronounced cellular and spatial heterogeneity, and identifying key tumor subsets characterized by MET or NGFR expression. Notably, spatial profiling of tumors progressing under ICi or BRAFi therapy identified a population of BZW2+ tumor cells that promotes an immunosuppressive microenvironment, thereby impeding immune cell infiltration and intratumoral dispersion. Elevated BZW(2) expression was associated with ICi resistance and inversely correlated with the antigen transporter TAP1 and the activation of interferon/STAT1 signaling. Spatially aware automated profiling of immune-enriched niches distinguished hot niches, characterized by tumor expression of TAP1, PD-L2 and HLA-DRA from cold zones, which were characterized by high levels of BZW2 and the transcriptional regulator SOX4. This translational suppressor co-localized with the clinically targetable MET receptor in a subset of tumor cells exhibiting distinct immune evasion features. Finally, multiplex imaging based on MACSima showed that, across all tumors, only 0.1% of CD8A(+) T cells were in an activated state. |
| Keywords: | Spatial Biology, Brain Metastases, Melanoma, Immune Suppressive Programs |
| Source: | medRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2025.08.29.25334740v3 |
| Date: | 3 June 2026 |
| Official Publication: | https://doi.org/10.1101/2025.08.29.25334740 |
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