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| Item Type: | Article |
|---|---|
| Title: | Comprehensive analysis of B cell repopulation in ocrelizumab-treated patients with multiple sclerosis by mass cytometry and proteomics |
| Creators Name: | Wang, M., Otto, C., Fernández Zapata, C., Dehlinger, A., Gallaccio, G., Diekmann, L.M., Niederschweiberer, M., Schindler, P., Körtvélyessy, P., Kunkel, D., Paul, F., Ruprecht, K. and Böttcher, C. |
| Abstract: | Ocrelizumab, an anti-CD20 antibody, depletes CD20+ B cells, which subsequently repopulate over months. Little is known about changes in other immune cell populations and molecular markers associated with B cell repopulation. Here, we performed a comprehensive characterization of immune cells from ocrelizumab-treated patients with multiple sclerosis (MS) using mass cytometry. About 50% of patients showed naive B cell repopulation after 6 months mainly with a transitional phenotype, whereas CD27+ memory B cells only rarely repopulated. This repopulation was associated with a reduction of memory T cells and activated myeloid cells, as well as reduced expression of activation/migration markers in both cell types. A plasma proteomics analysis identified proteins including TNFRSF13C, associated with B cell depletion and repopulation. Plasma levels of neurofilament light-chain protein declined after ocrelizumab treatment was not linked with B cell repopulation. These findings identify potential soluble markers for monitoring of ocrelizumab treatment in MS. |
| Source: | iScience |
| ISSN: | 2589-0042 |
| Publisher: | Elsevier / Cell Press |
| Volume: | 28 |
| Number: | 5 |
| Page Range: | 112383 |
| Number of Pages: | 1 |
| Date: | 16 May 2025 |
| Official Publication: | https://doi.org/10.1016/j.isci.2025.112383 |
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