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| Item Type: | Preprint |
|---|---|
| Title: | Complex human hear bearing skin organoids reveal cell type specific susceptibility and innate immune responses to Herpes Simplex Virus 1 |
| Creators Name: | Wyler, Emanuel, Albertini, Silvia, Friedel, Caroline C., Manukyan, Artür, Winterfeldt, Annalena, Bracker, Nina, Huang, Jiabin, Krasemann, Susanne, Plumbon, Izabela, Altmüller, Janine, Conrad, Thomas, Reimer, Rudolph, Schneider, Carola, Radbruch, Helena, Hafezi, Wali, Hansen, Arne, Grundhoff, Adam, Landthaler, Markus, Fischer, Nicole and Czech-Sioli, Manja |
| Abstract: | Epithelial surfaces are the initial site of Herpes Simplex Virus 1 (HSV-1) infection. After latency establishment in the peripheral nervous system, reactivation can lead to skin pathologies. To reflect the complexity of skin we used complex human hair bearing skin organoids as a new model system for HSV-1 infection. We analyzed spread of the infection through the three-dimensional tissue and the cell type specific host response in a spatial temporal manner. Single-cell-resolution spatial transcriptomics and imaging shows a restricted viral infection in keratinocytes of the epidermis and hair follicles. The host factor IER3 is upregulated in papillary fibroblasts of the dermis as the infection progresses through the tissue. In keratinocytes of the stratum basale, we find IRF3 upregulated in infected but also virus negative cells, suggesting a signaling mechanism to initiate antiviral responses. Furthermore, we find a cell type specific inflammatory response with an upregulation of TNFSF9 in dermal fibroblasts and TNF in keratinocytes. TNFα target genes are induced in neighboring uninfected cells suggesting the induction of a localized inflammatory reaction to suppress viral infection. In summary, our findings position human hair-bearing skin organoids as a highly physiological model system for studying HSV-1 pathogenesis and reveal spatially organized host responses. |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2025.02.10.637415v2 |
| Date: | 12 January 2026 |
| Additional Information: | Accession "GSE313919" is currently private and is scheduled to be released on Dec 01, 2026. |
| Official Publication: | https://doi.org/10.1101/2025.02.10.637415 |
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