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Automated two-dimensional spatiotemporal analysis of mobile single-molecule FRET probes

Item Type:Video
Title:Automated two-dimensional spatiotemporal analysis of mobile single-molecule FRET probes
Creators Name:Schrangl, L., Göhring, J., Kellner, F., Huppa, J.B. and Schütz, G.J.
Abstract:This article presents a method for spatiotemporal analysis of mobile, single-molecule Förster resonance energy transfer (smFRET)-based probes using widefield fluorescence microscopy. The newly developed software toolkit allows the determination of smFRET time traces of moving probes, including the correct FRET efficiency and the molecular positions, as functions of time.
Keywords:Fluorescence Resonance Energy Transfer, Nanotechnology, Single Molecule Imaging, Software, Spatio-Temporal Analysis
Source:Journal of Visualized Experiments
ISSN:1940-087X
Publisher:JoVE
Number:177
Date:23 November 2021
Official Publication:https://doi.org/10.3791/63124-v
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