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| Item Type: | Video |
|---|---|
| Title: | Automated two-dimensional spatiotemporal analysis of mobile single-molecule FRET probes |
| Creators Name: | Schrangl, L., Göhring, J., Kellner, F., Huppa, J.B. and Schütz, G.J. |
| Abstract: | This article presents a method for spatiotemporal analysis of mobile, single-molecule Förster resonance energy transfer (smFRET)-based probes using widefield fluorescence microscopy. The newly developed software toolkit allows the determination of smFRET time traces of moving probes, including the correct FRET efficiency and the molecular positions, as functions of time. |
| Keywords: | Fluorescence Resonance Energy Transfer, Nanotechnology, Single Molecule Imaging, Software, Spatio-Temporal Analysis |
| Source: | Journal of Visualized Experiments |
| ISSN: | 1940-087X |
| Publisher: | JoVE |
| Number: | 177 |
| Date: | 23 November 2021 |
| Official Publication: | https://doi.org/10.3791/63124-v |
| Related to: |
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