Helmholtz Gemeinschaft


GIPC1 regulates MACC1-driven metastasis

PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
[img] Other (Supplementary Material)

Item Type:Article
Title:GIPC1 regulates MACC1-driven metastasis
Creators Name:Siegel, F. and Schmidt, H. and Juneja, M. and Smith, J. and Herrmann, P. and Kobelt, D. and Sharma, K. and Fichtner, I. and Walther, W. and Dittmar, G. and Volkmer, R. and Rathjen, F.G. and Schlag, P.M. and Stein, U.
Abstract:BACKGROUND: Identification of cancer metastasis-relevant molecular networks is desired to provide the basis for understanding and developing intervention strategies. Here we address the role of GIPC1 in the process of MACC1-driven metastasis. MACC1 is a prognostic indicator for patient metastasis formation and metastasis-free survival. MACC1 controls gene transcription, promotes motility, invasion and proliferation of colon cancer cells in vitro, and causes tumor growth and metastasis in mice. METHODS: By using yeast-two-hybrid assay, mass spectrometry, co-immunoprecipitation and peptide array we analyzed GIPC1 protein binding partners, by using the MACC1 gene promoter and chromatin immunoprecipitation and electrophoretic mobility shift assay we probed for GIPC1 as transcription factor. We employed GIPC1/MACC1-manipulated cell lines for in vitro and in vivo analyses, and we probed the GIPC1/MACC1 impact using human primary colorectal cancer (CRC) tissue. RESULTS: We identified MACC1 and its paralogue SH3BP4 as protein binding partners of the protein GIPC1, and we also demonstrated the binding of GIPC1 as transcription factor to the MACC1 promoter (TSS to -60 bp). GIPC1 knockdown reduced endogenous, but not CMV promoter-driven MACC1 expression, and diminished MACC1-induced cell migration and invasion. GIPC1 suppression reduced tumor growth and metastasis in mice intrasplenically transplanted with MACC1-overexpressing CRC cells. In human primary CRC specimens, GIPC1 correlates with MACC1 expression and is of prognostic value for metastasis formation and metastasis-free survival. Combination of MACC1 and GIPC1 expression improved patient survival prognosis, whereas SH3BP4 expression did not show any prognostic value. CONCLUSIONS: We identified an important, dual function of GIPC1 - as protein interaction partner and as transcription factor of MACC1 – for tumor progression and cancer metastasis.
Keywords:MACC1, GIPC1, Protein-Protein Interaction, Transcription Factor, Patient Survival Prognosis, Animals, Mice
Source:Frontiers in Oncology
Publisher:Frontiers Media SA
Page Range:1280977
Date:8 December 2023
Official Publication:https://doi.org/10.3389/fonc.2023.1280977
PubMed:View item in PubMed

Repository Staff Only: item control page


Downloads per month over past year

Open Access
MDC Library