Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders

Item Type:	Article
Title:	Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders
Creators Name:	Eckl, K.M. and Weindl, G. and Ackermann, K. and Küchler, S. and Casper, R. and Radowski, M.R. and Haag, R. and Hennies, H.C. and Schäfer-Korting, M.
Abstract:	The aim of this study was to assess a recently established 3D model of congenital ichthyosis, representing severe epidermal barrier function defects, for skin penetration and permeation. We have generated disease models by knock-down of either TGM1 or ALOXE3 in primary human keratinocytes, and using keratinocytes and fibroblasts from patients with congenital ichthyosis. The results indicate disturbed barrier function as demonstrated by increased permeation of testosterone and caffeine particularly in TGM1 knock-down models compared to control models. In addition, enhanced penetration of the model dye nile red incorporated into solid lipid nanoparticles and core-multishell nanotransporters, respectively, was evident in disease models. Thus, in vitro skin disease models reproduce differences in barrier permeability and function seen in congenital ichthyosis and pave the way to personalised disease models. Furthermore, our findings indicate that nanocarriers may be useful in new, topical therapeutic approaches for the currently very limited treatment of congenital ichthyosis.
Keywords:	Epidermal Barrier, Keratinization Disorder, Nanoparticles, Skin Disease Models, Skin Permeation and Penetration, Animals, Mice
Source:	Experimental Dermatology
ISSN:	0906-6705
Publisher:	Wiley
Volume:	23
Number:	4
Page Range:	286-288
Date:	April 2014
Official Publication:	https://doi.org/10.1111/exd.12366
PubMed:	View item in PubMed

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