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Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root

Item Type:Article
Title:Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root
Creators Name:Nolan, T.M. and Vukašinović, N. and Hsu, C.W. and Zhang, J. and Vanhoutte, I. and Shahan, R. and Taylor, I.W. and Greenstreet, L. and Heitz, M. and Afanassiev, A. and Wang, P. and Szekely, P. and Brosnan, A. and Yin, Y. and Schiebinger, G. and Ohler, U. and Russinova, E. and Benfey, P.N.
Abstract:Brassinosteroids are plant steroid hormones that regulate diverse processes, such as cell division and cell elongation, through gene regulatory networks that vary in space and time. By using time series single-cell RNA sequencing to profile brassinosteroid-responsive gene expression specific to different cell types and developmental stages of the Arabidopsis root, we identified the elongating cortex as a site where brassinosteroids trigger a shift from proliferation to elongation associated with increased expression of cell wall-related genes. Our analysis revealed HOMEOBOX FROM ARABIDOPSIS THALIANA 7 (HAT7) and GT-2-LIKE 1 (GTL1) as brassinosteroid-responsive transcription factors that regulate cortex cell elongation. These results establish the cortex as a site of brassinosteroid-mediated growth and unveil a brassinosteroid signaling network regulating the transition from proliferation to elongation, which illuminates aspects of spatiotemporal hormone responses.
Keywords:Arabidopsis, Arabidopsis Proteins, Brassinosteroids, Gene Regulatory Networks, Plant Gene Expression Regulation, Plant Growth Regulators, Transcription Factors
Publisher:American Association for the Advancement of Science
Page Range:eadf4721
Date:31 March 2023
Additional Information:Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
Official Publication:https://doi.org/10.1126/science.adf4721
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

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