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Serum starvation accelerates intracellular metabolism in endothelial cells

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Item Type:Article
Title:Serum starvation accelerates intracellular metabolism in endothelial cells
Creators Name:Lorenz, M. and Fritsche-Guenther, R. and Bartsch, C. and Vietzke, A. and Eisenberger, A. and Stangl, K. and Stangl, V. and Kirwan, J.A.
Abstract:Periods of low energy supply are challenging conditions for organisms and cells during fasting or famine. Although changes in nutrient levels in the blood are first sensed by endothelial cells, studies on their metabolic adaptations to diminished energy supply are lacking. We analyzed the dynamic metabolic activity of human umbilical vein endothelial cells (HUVECs) in basal conditions and after serum starvation. Metabolites of glycolysis, the tricarboxylic acid (TCA) cycle, and the glycerol pathway showed lower levels after serum starvation, whereas amino acids had increased levels. A metabolic flux analysis with (13)C-glucose or (13)C-glutamine labeling for different time points reached a plateau phase of incorporation after 30 h for (13)C-glucose and after 8 h for (13)C-glutamine under both experimental conditions. Notably, we observed a faster label incorporation for both (13)C-glucose and (13)C-glutamine after serum starvation. In the linear range of label incorporation after 3 h, we found a significantly faster incorporation of central carbon metabolites after serum starvation compared to the basal state. These findings may indicate that endothelial cells develop increased metabolic activity to cope with energy deficiency. Physiologically, it can be a prerequisite for endothelial cells to form new blood vessels under unfavorable conditions during the process of angiogenesis in vivo.
Keywords:Metabolism, Serum Starvation, Endothelial Cells, HUVEC, Flux Analysis, Labeling
Source:International Journal of Molecular Sciences
Page Range:1189
Date:7 January 2023
Official Publication:https://doi.org/10.3390/ijms24021189
PubMed:View item in PubMed

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