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Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions

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Item Type:Article
Title:Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
Creators Name:Giacomello, G. and Böttcher, C. and Parr, M.K.
Abstract:Differences in metabolic profiles can link to functional changes of immune cells in disease conditions. Here, we detail a protocol for the detection and quantitation of 19 metabolites in one analytical run. We provide the parameters for chromatographic separation and mass spectrometric analysis of isotopically labeled and unlabeled metabolites. We include steps for incubation and sample preparation of PBMCs and monocytes. This protocol overcomes the chromatographic challenges caused by the chelating properties of some metabolites.
Keywords:Chemistry, Immunology, Mass Spectrometry, Metabolism, Metabolomics
Source:STAR Protocols
ISSN:2666-1667
Publisher:Cell Press / Elsevier
Volume:3
Number:4
Page Range:101715
Date:16 December 2022
Official Publication:http://doi.org/10.1016/j.xpro.2022.101715
PubMed:View item in PubMed

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