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| Item Type: | Article |
|---|---|
| Title: | Analysis of single-cell transcriptome data in drosophila |
| Creators Name: | Yousefian, S., Musillo, M.J. and Bageritz, J. |
| Abstract: | The fly Drosophila is a versatile model organism that has led to fascinating biological discoveries. In the past few years, Drosophila researchers have used single-cell RNA-sequencing (scRNA-seq) to gain insights into the cellular composition, and developmental processes of various tissues and organs. Given the success of single-cell technologies a variety of computational tools and software packages were developed to enable and facilitate the analysis of scRNA-seq data. In this book chapter we want to give guidance on analyzing droplet-based scRNA-seq data from Drosophila. We will initially describe the preprocessing commonly done for Drosophila, point out possible downstream analyses, and finally highlight computational methods developed using Drosophila scRNA-seq data. |
| Keywords: | Single-Cell RNA-Seq, Data Analysis, Seurat, Computational Methods, Droplet Based, Software Packages, Animals, Drosophila |
| Source: | Methods in Molecular Biology |
| Series Name: | Methods in Molecular Biology |
| Title of Book: | Drosophila |
| ISSN: | 1064-3745 |
| ISBN: | 9781071625408 |
| Publisher: | Springer / Humana Press |
| Volume: | 2540 |
| Number: | 3 |
| Page Range: | 93-111 |
| Number of Pages: | 0 |
| Date: | 19 August 2022 |
| Official Publication: | https://doi.org/10.1007/978-1-0716-2541-5_4 |
| PubMed: | View item in PubMed |
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