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| Item Type: | Article |
|---|---|
| Title: | Generation of redirected engineered human chimeric antigen receptor (CAR) T cells |
| Creators Name: | Bunse, M. and Höpken, U.E. |
| Abstract: | Chimeric antigen receptor (CAR) T cell therapy that involves genetic engineering a patient's own immune cells with antigen-specific receptors has shown remarkable efficacy in blood cancer treatment. Numerous clinical studies with CAR T cells targeting the blood cell surface protein CD19 led to the FDA 's first approval of a genetically engineered cell therapy. The process of generating potent CAR T cells involves several carefully performed manufacturing steps. Here, we describe the generation of redirected engineered human CAR T cells for preclinical studies starting with the CAR design, retroviral gene transfer, detection of CAR expression, and expansion of transduced T cells. |
| Keywords: | Chimeric Antigen Receptor, T Cells, Retroviral Transduction, Tumor Immunotherapy |
| Source: | Methods in Molecular Biology |
| Series Name: | Methods in Molecular Biology |
| Title of Book: | Gene therapy of cancer : methods and protocols |
| ISSN: | 1064-3745 |
| ISBN: | 9781071624401 |
| Publisher: | Springer / Humana Press |
| Volume: | 2521 |
| Number: | 3 |
| Page Range: | 67-83 |
| Number of Pages: | 0 |
| Date: | 23 June 2022 |
| Official Publication: | https://doi.org/10.1007/978-1-0716-2441-8_4 |
| PubMed: | View item in PubMed |
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