Modulation of RNA polymerase assembly dynamics in transcriptional regulation

Item Type:	Article
Title:	Modulation of RNA polymerase assembly dynamics in transcriptional regulation
Creators Name:	Gorski, S.A. and Snyder, S.K. and John, S. and Grummt, I. and Misteli, T.
Abstract:	The interaction of transcription factors with target genes is highly dynamic. Whether the dynamic nature of these interactions is merely an intrinsic property of transcription factors or serves a regulatory role is unknown. Here we have used single-cell fluorescence imaging combined with computational modeling and chromatin immunoprecipitation to analyze transcription complex dynamics in gene regulation during the cell cycle in living cells. We demonstrate a link between the dynamics of RNA polymerase I (RNA Pol I) assembly and transcriptional output. We show that transcriptional upregulation is accompanied by prolonged retention of RNA Pol I components at the promoter, resulting in longer promoter dwell time, and an increase in the steady-state population of assembling polymerase. As a consequence, polymerase assembly efficiency and, ultimately, the rate of entry into processive elongation are elevated. Our results show that regulation of rDNA transcription in vivo occurs via modulation of the efficiency of transcription complex subunit capture and assembly.
Keywords:	Cell Cycle, Cultured Cells, Fluorescence Recovery after Photobleaching, Gene Expression Regulation, Genetic Promoter Regions, Genetic Transcription, Protein Subunits, Ribosomal DNA, RNA Polymerase I, Animals
Source:	Molecular Cell
ISSN:	1097-2765
Publisher:	Cell Press
Volume:	30
Number:	4
Page Range:	486-497
Date:	23 May 2008
Official Publication:	https://doi.org/10.1016/j.molcel.2008.04.021
PubMed:	View item in PubMed

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