![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
|
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
6MB | |
![[img]](http://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplemental Information)
238MB |
| Item Type: | Article | ||||
|---|---|---|---|---|---|
| Title: | Single-cell-resolved dynamics of chromatin architecture delineate cell and regulatory states in zebrafish embryos | ||||
| Creators Name: | McGarvey, A.C. and Kopp, W. and Vučićević, D. and Mattonet, K. and Kempfer, R. and Hirsekorn, A. and Bilić, I. and Gil, M. and Trinks, A. and Merks, A.M. and Panáková, D. and Pombo, A. and Akalin, A. and Junker, J.P. and Stainier, D.Y.R. and Garfield, D. and Ohler, U. and Lacadie, S.A. | ||||
| Abstract: | DNA accessibility of cis-regulatory elements (CREs) dictates transcriptional activity and drives cell differentiation during development. While many genes regulating embryonic development have been identified, the underlying CRE dynamics controlling their expression remain largely uncharacterized. To address this, we produced a multimodal resource and genomic regulatory map for the zebrafish community, which integrates single-cell combinatorial indexing assay for transposase-accessible chromatin with high-throughput sequencing (sci-ATAC-seq) with bulk histone PTMs and Hi-C data to achieve a genome-wide classification of the regulatory architecture determining transcriptional activity in the 24-h post-fertilization (hpf) embryo. We characterized the genome-wide chromatin architecture at bulk and single-cell resolution, applying sci-ATAC-seq on whole 24-hpf stage zebrafish embryos, generating accessibility profiles for ∼23,000 single nuclei. We developed a genome segmentation method, ScregSeg (single-cell regulatory landscape segmentation), for defining regulatory programs, and candidate CREs, specific to one or more cell types. We integrated the ScregSeg output with bulk measurements for histone post-translational modifications and 3D genome organization and identified new regulatory principles between chromatin modalities prevalent during zebrafish development. Sci-ATAC-seq profiling of npas4l/cloche mutant embryos identified novel cellular roles for this hematovascular transcriptional master regulator and suggests an intricate mechanism regulating its expression. Our work defines regulatory architecture and principles in the zebrafish embryo and establishes a resource of cell-type-specific genome-wide regulatory annotations and candidate CREs, providing a valuable open resource for genomics, developmental, molecular, and computational biology. | ||||
| Keywords: | Chromatin, Cis Regulatory Element, Enhancer, Promoter, Single-Cell ATAC-seq, Hi-C, npas4l, Animals, Zebrafish | ||||
| Source: | Cell Genomics | ||||
| ISSN: | 2666-979X | ||||
| Publisher: | Cell Press | ||||
| Volume: | 2 | ||||
| Number: | 1 | ||||
| Page Range: | 100083 | ||||
| Date: | 13 January 2022 | ||||
| Official Publication: | https://doi.org/10.1016/j.xgen.2021.100083 | ||||
| PubMed: | View item in PubMed | ||||
| Related to: |
|
Repository Staff Only: item control page
Download Statistics
Download Statistics
