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Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension

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Item Type:Article
Title:Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension
Creators Name:Dahale, S. and Ruiz-Orera, J. and Silhavy, J. and Hübner, N. and van Heesch, S. and Pravenec, M. and Atanur, S.S.
Abstract:The role of alternative promoter usage in tissue-specific gene expression has been well established; however, its role in complex diseases is poorly understood. We performed cap analysis of gene expression (CAGE) sequencing from the left ventricle of a rat model of hypertension, the spontaneously hypertensive rat (SHR), and a normotensive strain, Brown Norway to understand the role of alternative promoter usage in complex disease. We identified 26,560 CAGE-defined transcription start sites in the rat left ventricle, including 1,970 novel cardiac transcription start sites. We identified 28 genes with alternative promoter usage between SHR and Brown Norway, which could lead to protein isoforms differing at the amino terminus between two strains and 475 promoter switching events altering the length of the 5' UTR. We found that the shift in Insr promoter usage was significantly associated with insulin levels and blood pressure within a panel of HXB/BXH recombinant inbred rat strains, suggesting that hyperinsulinemia due to insulin resistance might lead to hypertension in SHR. Our study provides a preliminary evidence of alternative promoter usage in complex diseases.
Keywords:Animals, Rats
Source:Life Science Alliance
ISSN:2575-1077
Publisher:Life Science Alliance
Volume:5
Number:4
Page Range:e202101234
Date:April 2022
Official Publication:https://doi.org/10.26508/lsa.202101234
PubMed:View item in PubMed

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