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| Item Type: | Article |
|---|---|
| Title: | Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly |
| Creators: |
Cereghetti, G.  ORCID: https://orcid.org/0000-0001-7732-9435, Wilson-Zbinden, C., Kissling, V.M. ORCID: https://orcid.org/0000-0002-4524-9905, Diether, M., Arm, A., Yoo, H., Piazza, I. ORCID: https://orcid.org/0000-0001-5895-6134, Saad, S. ORCID: https://orcid.org/0000-0003-1062-2906, Picotti, P., Drummond, D.A. ORCID: https://orcid.org/0000-0001-7018-7059, Sauer, U., Dechant, R. ORCID: https://orcid.org/0000-0002-4094-4773 and Peter, M. ORCID: https://orcid.org/0000-0002-2160-6824
|
| Abstract: | Cells respond to stress by blocking translation, rewiring metabolism and forming transient messenger ribonucleoprotein assemblies called stress granules (SGs). After stress release, re-establishing homeostasis and disassembling SGs requires ATP-consuming processes. However, the molecular mechanisms whereby cells restore ATP production and disassemble SGs after stress remain poorly understood. Here we show that upon stress, the ATP-producing enzyme Cdc19 forms inactive amyloids, and that their rapid re-solubilization is essential to restore ATP production and disassemble SGs in glucose-containing media. Cdc19 re-solubilization is initiated by the glycolytic metabolite fructose-1,6-bisphosphate, which directly binds Cdc19 amyloids, allowing Hsp104 and Ssa2 chaperone recruitment and aggregate re-solubilization. Fructose-1,6-bisphosphate then promotes Cdc19 tetramerization, which boosts its activity to further enhance ATP production and SG disassembly. Together, these results describe a molecular mechanism that is critical for stress recovery and directly couples cellular metabolism with SG dynamics via the regulation of reversible Cdc19 amyloids. |
| Keywords: | Adenosine Triphosphate, Amyloid, Cell Cycle Proteins, Cytoplasmic Granules, Fructosediphosphates, HSP70 Heat-Shock Proteins, Heat-Shock Proteins, Physiological Stress, Pyruvate Kinase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins |
| Source: | Nature Cell Biology |
| ISSN: | 1465-7392 |
| Publisher: | Nature Publishing Group |
| Volume: | 23 |
| Number: | 10 |
| Page Range: | 1085-1094 |
| Date: | October 2021 |
| Additional Information: | Erratum in: Nat Cell Biol (2021). |
| Official Publication: | https://doi.org/10.1038/s41556-021-00760-4 |
| PubMed: | View item in PubMed |
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