Human brain organoids assemble functionally integrated bilateral optic vesicles

Item Type:	Article
Title:	Human brain organoids assemble functionally integrated bilateral optic vesicles
Creators Name:	Gabriel, E. and Albanna, W. and Pasquini, Gi. and Ramani, A. and Josipovic, N. and Mariappan, A. and Schinzel, F. and Karch, C.M. and Bao, G. and Gottardo, M. and Suren, A.A. and Hescheler, J. and Nagel-Wolfrum, K. and Persico, V. and Rizzoli, S.O. and Altmüller, J. and Riparbelli, M.G. and Callaini, G. and Goureau, O. and Papantonis, A. and Busskamp, V. and Schneider, T. and Gopalakrishnan, J.
Abstract:	During embryogenesis, optic vesicles develop from the diencephalon via a multistep process of organogenesis. Using induced pluripotent stem cell (iPSC)-derived human brain organoids, we attempted to simplify the complexities and demonstrate formation of forebrain-associated bilateral optic vesicles, cellular diversity, and functionality. Around day 30, brain organoids attempt to assemble optic vesicles, which develop progressively as visible structures within 60 days. These optic vesicle-containing brain organoids (OVB-organoids) constitute a developing optic vesicle's cellular components, including primitive corneal epithelial and lens-like cells, retinal pigment epithelia, retinal progenitor cells, axon-like projections, and electrically active neuronal networks. OVB-organoids also display synapsin-1, CTIP-positive myelinated cortical neurons, and microglia. Interestingly, various light intensities could trigger photosensitive activity of OVB-organoids, and light sensitivities could be reset after transient photobleaching. Thus, brain organoids have the intrinsic ability to self-organize forebrain-associated primitive sensory structures in a topographically restricted manner and can allow interorgan interaction studies within a single organoid.
Keywords:	Brain Organoids, Optic Vesicles, Forebrain Organoids, iPSCs, Primordial Eye Fields, OVB-Organoids, FOXG1, Primary Cilium, Retinal Pigment Epithelium
Source:	Cell Stem Cell
ISSN:	1934-5909
Publisher:	Cell Press
Volume:	28
Number:	10
Page Range:	1740-1757.e8
Date:	October 2021
Official Publication:	https://doi.org/10.1016/j.stem.2021.07.010
PubMed:	View item in PubMed

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