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Regional specialization and fate specification of bone stromal cells in skeletal development

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Item Type:Article
Title:Regional specialization and fate specification of bone stromal cells in skeletal development
Creators Name:Sivaraj, K.K. and Jeong, H.W. and Dharmalingam, B. and Zeuschner, D. and Adams, S. and Potente, M. and Adams, R.H.
Abstract:Bone stroma contributes to the regulation of osteogenesis and hematopoiesis but also to fracture healing and disease processes. Mesenchymal stromal cells from bone (BMSCs) represent a heterogenous mixture of different subpopulations with distinct molecular and functional properties. The lineage relationship between BMSC subsets and their regulation by intrinsic and extrinsic factors are not well understood. Here, we show with mouse genetics, ex vivo cell differentiation assays, and transcriptional profiling that BMSCs from metaphysis (mpMSCs) and diaphysis (dpMSCs) are fundamentally distinct. Fate-tracking experiments and single-cell RNA sequencing indicate that bone-forming osteoblast lineage cells and dpMSCs, including leptin receptor-positive (LepR(+)) reticular cells in bone marrow, emerge from mpMSCs in the postnatal metaphysis. Finally, we show that BMSC fate is controlled by platelet-derived growth factor receptor β (PDGFRβ) signaling and the transcription factor Jun-B. The sum of our findings improves our understanding of BMSC development, lineage relationships, and differentiation.
Keywords:Bone Mesenchymal Stromal Cells, Metaphysis, Diaphysis, Osteoblast Lineage Cells, Adipocytes, Mouse Genetics, Single-Cell RNA Sequencing, Animals, Mice
Source:Cell Reports
Publisher:Cell Press / Elsevier
Page Range:109352
Date:13 July 2021
Official Publication:https://doi.org/10.1016/j.celrep.2021.109352
PubMed:View item in PubMed

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