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Structure of the Yersinia injectisome in intracellular host cell phagosomes revealed by cryo FIB electron tomography

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Item Type:Article
Title:Structure of the Yersinia injectisome in intracellular host cell phagosomes revealed by cryo FIB electron tomography
Creators Name:Berger, C. and Ravelli, R.B.G. and López-Iglesias, C. and Kudryashev, M. and Diepold, A. and Peters, P.J.
Abstract:Many pathogenic bacteria use the type III secretion system (T3SS), or injectisome, to secrete toxins into host cells. These protruding systems are primary targets for drug and vaccine development. Upon contact between injectisomes and host membranes, toxin secretion is triggered. How this works structurally and functionally is yet unknown. Using cryo-focused ion beam milling and cryo-electron tomography, we visualized injectisomes of Yersinia enterocolitica inside the phagosomes of infected human myeloid cells in a close-to-native state. We observed that a minimum needle length is required for injectisomes to contact the host membrane and bending of host membranes by some injectisomes that contact the host. Through subtomogram averaging, the structure of the entire injectisome was determined, which revealed structural differences in the cytosolic sorting platform compared to other bacteria. These findings contribute to understanding how injectisomes secrete toxins into host cells and provides the indispensable native context. The application of these cryo-electron microscopy techniques paves the way for the study of the 3D structure of infection-relevant protein complexes in host-pathogen interactions.
Keywords:Type III Secretion System, Cryo-Electron Tomography, Cryo-Focused Ion Beam Lamella, Subtomogram Averaging, Yersinia Enterocolitica, Bacterial Adhesin YadA
Source:Journal of Structural Biology
Page Range:107701
Date:March 2021
Official Publication:https://doi.org/10.1016/j.jsb.2021.107701
PubMed:View item in PubMed

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