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| Item Type: | Article |
|---|---|
| Title: | Dual-view light-sheet imaging through a tilted glass interface using a deformable mirror |
| Creators Name: | Vladimirov, N., Preusser, F., Wisniewski, J., Yaniv, Z., Desai, R.A., Woehler, A. and Preibisch, S. |
| Abstract: | Light-sheet microscopy has become indispensable for imaging developing organisms, and imaging from multiple directions (views) is essential to improve its spatial resolution. We combine multi-view light-sheet microscopy with microfluidics using adaptive optics (deformable mirror) which corrects aberrations introduced by the 45°-tilted glass coverslip. The optimal shape of the deformable mirror is computed by an iterative algorithm that optimizes the point-spread function in two orthogonal views. Simultaneous correction in two optical arms is achieved via a knife-edge mirror that splits the excitation path and combines the detection paths. Our design allows multi-view light-sheet microscopy with microfluidic devices for precisely controlled experiments and high-content screening. |
| Keywords: | Animals, Caenorhabditis elegans |
| Source: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Publisher: | Optical Society of America |
| Volume: | 12 |
| Number: | 4 |
| Page Range: | 2186-2203 |
| Date: | 1 April 2021 |
| Official Publication: | https://doi.org/10.1364/BOE.416737 |
| PubMed: | View item in PubMed |
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