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Ribosome levels selectively regulate translation and lineage commitment in human hematopoiesis

Item Type:Article
Title:Ribosome levels selectively regulate translation and lineage commitment in human hematopoiesis
Creators Name:Khajuria, R.K. and Munschauer, M. and Ulirsch, J.C. and Fiorini, C. and Ludwig, L.S. and McFarland, S.K. and Abdulhay, N.J. and Specht, H. and Keshishian, H. and Mani, D.R. and Jovanovic, M. and Ellis, S.R. and Fulco, C.P. and Engreitz, J.M. and Schütz, S. and Lian, J. and Gripp, K.W. and Weinberg, O.K. and Pinkus, G.S. and Gehrke, L. and Regev, A. and Lander, E.S. and Gazda, H.T. and Lee, W.Y. and Panse, V.G. and Carr, S.A. and Sankaran, V.G.
Abstract:Blood cell formation is classically thought to occur through a hierarchical differentiation process, although recent studies have shown that lineage commitment may occur earlier in hematopoietic stem and progenitor cells (HSPCs). The relevance to human blood diseases and the underlying regulation of these refined models remain poorly understood. By studying a genetic blood disorder, Diamond-Blackfan anemia (DBA), where the majority of mutations affect ribosomal proteins and the erythroid lineage is selectively perturbed, we are able to gain mechanistic insight into how lineage commitment is programmed normally and disrupted in disease. We show that in DBA, the pool of available ribosomes is limited, while ribosome composition remains constant. Surprisingly, this global reduction in ribosome levels more profoundly alters translation of a select subset of transcripts. We show how the reduced translation of select transcripts in HSPCs can impair erythroid lineage commitment, illuminating a regulatory role for ribosome levels in cellular differentiation.
Keywords:Hematopoiesis, Lineage Commitment, GATA1, Genetics, Ribosome, Translation, Diamond-Blackfan Anemia, Erythropoiesis
Publisher:Cell Press
Page Range:90-103
Date:22 March 2018
Official Publication:https://doi.org/10.1016/j.cell.2018.02.036
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

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