![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
|
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
2MB | |
![[img]](http://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplementary Data)
4MB |
| Item Type: | Article |
|---|---|
| Title: | Comprehensive analysis of translation from overexpressed circular RNAs reveals pervasive translation from linear transcripts |
| Creators Name: | Ho-Xuan, H. and Glažar, P. and Latini, C. and Heizler, K. and Haase, J. and Hett, R. and Anders, M. and Weichmann, F. and Bruckmann, A. and Van den Berg, D. and Hüttelmaier, S. and Rajewsky, N. and Hackl, C. and Meister, G. |
| Abstract: | Circular RNAs (circRNAs) encompass a widespread and conserved class of RNAs, which are generated by back-splicing of downstream 5' to upstream 3' splice sites. CircRNAs are tissue-specific and have been implicated in diseases including cancer. They can function as sponges for microRNAs (miRNAs) or RNA binding proteins (RBPs), for example. Moreover, some contain open reading frames (ORFs) and might be translated. The functional relevance of such peptides, however, remains largely elusive. Here, we report that the ORF of circZNF609 is efficiently translated when expressed from a circZNF609 overexpression construct. However, endogenous proteins could not be detected. Moreover, initiation of circZNF609 translation is independent of m(6)A-generating enzyme METTL3 or RNA sequence elements such as internal ribosome entry sites (IRESs). Surprisingly, a comprehensive mutational analysis revealed that deletion constructs, which are deficient in producing circZNF609, still generate the observed protein products. This suggests that the apparent circZNF609 translation originates from trans-splicing by-products of the overexpression plasmids and underline that circRNA overexpression constructs need to be evaluated carefully, particularly when functional studies are performed. |
| Keywords: | Base Sequence, Circular RNA, Gene Expression Regulation, HEK293 Cells, Internal Ribosome Entry Sites, Methyltransferases, MicroRNAs, Protein Biosynthesis, RNA Splice Sites, RNA-Binding Proteins |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 48 |
| Number: | 18 |
| Page Range: | 10368-10382 |
| Date: | 9 October 2020 |
| Official Publication: | https://doi.org/10.1093/nar/gkaa704 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
Download Statistics
Download Statistics
