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Isolation and long-term cultivation of mouse alveolar macrophages

Item Type:Article
Title:Isolation and long-term cultivation of mouse alveolar macrophages
Creators Name:Busch, C. J.-L. and Favret, J. and Geirsdóttir, L. and Molawi, K. and Sieweke, M.H.
Abstract:Alveolar macrophages (AM) are tissue-resident macrophages that colonize the lung around birth and can self-maintain long-term in an adult organism without contribution of monocytes. AM are located in the pulmonary alveoli and can be harvested by washing the lungs using the method of bronchoalveolar lavage (BAL). Here, we compared different conditions of BAL to obtain high yields of murine AM for in vitro culture and expansion of AM. In addition, we describe specific culture conditions, under which AM proliferate long-term in liquid culture in the presence of granulocyte-macrophage colony-stimulating factor. This method can be used to obtain large numbers of AM for in vivo transplantation or for in vitro experiments with primary mouse macrophages.
Keywords:Macrophage, Alveolar, Lungs, Self-Renewal, Bronchoalveolar Lavage, Primary Cell Culture, Animals, Mice
Source:Bio-protocol
ISSN:2331-8325
Publisher:Bio-protocol LLC
Volume:9
Number:14
Date:20 July 2019
Official Publication:https://doi.org/10.21769/BioProtoc.3302
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

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